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KMID : 0438219740110021183
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Korea University Medical Journal
1974 Volume.11 No. 2 p.1183 ~ p.1190
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Seasonal Variation of Vasopressin Action on Water Balance in the Intact Frog
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Abstract
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The rate of water uptake across the skin and urine formation by the kidneys were measured in the living frog, (Rana Temporaria), especially under the influence of vasopressin, when frogs were kept in distilled water at 22C. Adult frogs of both sexes weighing between 20 and 30 g were used. Experiments were performed in four seasons throughout the year. At the onset of the experiment polyethylene catheter introduced into cloaca through cloaca orifice, and fixed After recovery frogs were placed in 200 ml glass beakers containing 100 ml of distilled water, oxygen supplied through polyethylene tube into outside bathing water. The amount of water uptake was calculated from the changes in body weight per unit time and then expressed as % of initial body weight. Vasopressin, (Pitressin, Parke Davis and Co. ) 0.06 u/g B. W. diluted in 0.65% NaCl solution. The volume injected varied from 0.1 to 0.3 ml for frog. All injection were given into lymphatic sac.
The results obtained are summarized as follows:
1. The water uptake across the skin increased significantly after the injection of vasopressin in spring and autumn season whereas decreased in summer season.
2. Antidiuretic action of vasopressin by the kidneys were obtained in all the seasons except spring season.
3. Vasopressin promote the water uptake across the skin, water accumulated in the body steadly in the all seasons except summer season.
4. In summer, vasopressin upset the frog´s water balance, even when it did suppress urine output.
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